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Curriculum Details
- Bacterial Genetic Transformation
- Protein Purification: Column Chromatography
- DNA Restriction Analysis (DNA
Fingerprinting)
- Amplification and Analysis of
Human DNA by PCR
- Protein
Electrophoresis (SDS-PAGE)
- Bioinformatics
- Immunology
- DNA
Microarrays and Gene Expression
- RNAi
and Gene Silencing
DNA Restriction Analysis (DNA Fingerprinting)
How can scientists compare the similarities and differences between two different samples of DNA? How are restriction endonucleases used to create RFLPs (Random Fragment Length Polymorphisms)? How can DNA fragments of different lengths be separated and studied? What is a DNA Fingerprint? In this lab sequence, you will use several restriction enzymes to digest DNA samples for analysis by agarose gel electrophoresis. The resulting DNA Fingerprint will allow you to distinguish among several DNA samples.
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