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Curriculum Details
- Bacterial Genetic Transformation
- Protein Purification: Column Chromatography
- DNA Restriction Analysis (DNA
Fingerprinting)
- Amplification and Analysis of
Human DNA by PCR
- Protein
Electrophoresis (SDS-PAGE)
- Bioinformatics
- Immunology
- DNA
Microarrays and Gene Expression
- RNAi
and Gene Silencing
Protein Purification: Column Chromatography
In order to study a particular protein, it is often necessary to isolate it in pure form. How can scientists separate and purify a novel protein from a complex mixture of cellular components? In this lab sequence, you will learn bacterial culture techniques and how to isolate and purify an engineered bacterial protein using hydrophobic interaction column chromatography. A form of affinity chromatography, HIC separates proteins based on their ability to bind to specific chemical groups (referred to as ligand groups) attached to beads in a column matrix. You will also learn to separate a mixture of proteins using Size Exclusion Chromatography (SEC).
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