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Curriculum Details
- Bacterial Genetic Transformation
- Protein Purification: Column Chromatography
- DNA Restriction Analysis (DNA
Fingerprinting)
- Amplification and Analysis of
Human DNA by PCR
- Protein
Electrophoresis (SDS-PAGE)
- Bioinformatics
- Immunology
- DNA
Microarrays and Gene Expression
- RNAi
and Gene Silencing
Bacterial Genetic
Transformation
Vital proteins such as enzymes, hormones, and structural
molecules are coded by our DNA with amazing consistency.
What is the nature of the genetic code, and how is it
translated into the vast array of proteins we need to
live? How do scientists genetically engineer bacteria to
produce a novel protein? Learn to genetically alter the
phenotype of a wild-type strain of bacteria by inducing
the uptake of a genetically-engineered form of DNA called
a plasmid. This plasmid confers antibiotic resistance and
the ability to synthesize a novel protein product. You
will learn about operons and the molecular control
mechanisms by which genes are activated or silenced based
on the organism's need for specific proteins at any given
moment.
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